Lifting weights first, especially heavy weights, will tire you out enough that it might get difficult to do cardio later. So you may not be able to complete your workout and will not get the calorie-burning benefit of cardio. However, in theory, doing weights first would put your body into aerobic mode, and so by the time you get your cardio, you will already be in a fat burning state. If you are training with light weights, then this will help to increase your heart rate and prepare you for an intense cardio workout.
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